FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_14.351000000444fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_14.351000000444fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24563
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC463718.87798721654521RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT13205.373936408419167No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG1730.7043113626185726No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT1180.4803973456011074No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC300.1221349183731629RNA PCR Primer, Index 45 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4900.042.244945
GCCGTCT301.1077138E-437.49999645
ATGCCGT6450.032.09302544
CGTTCAT6600.031.70454428
AGCCCAC6600.031.70454417
AATCTCG6600.031.70454436
ACGTTCA6600.031.70454427
GAGCCCA6600.031.70454416
GACGTTC6600.031.70454426
AGACGTT6600.031.70454425
CGAGCCC6600.031.70454415
TCTCCGA6600.031.70454411
CTCCGAG6600.031.70454412
GCCCACG6550.031.60305418
CCACGAG6550.031.60305420
CACGAGA6550.031.60305421
CCCACGA6550.031.60305419
ACGAGAC6550.031.60305422
CGAGACG6550.031.60305423
GAGACGT6550.031.60305424
Produced by FastQC (version 0.11.2)