FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_13.351000000444d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_13.351000000444d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97018
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC17771.8316188748479665RNA PCR Primer, Index 45 (95% over 21bp)
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA2160.22263909789935887No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT1570.16182564060277474No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1310.13502648992970379No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATG1060.10925807582098167No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.10822733925663279No Hit
GTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1040.10719660269228391No Hit
GGACATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATA1040.10719660269228391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1100.042.95454445
ACCTGCA301.132234E-437.50000439
GACCTGC301.132234E-437.50000438
GGCTAGC301.132234E-437.5000042
AGCTTAG250.002097631836.016
TCTAGCG250.002097631836.09
CGGGGCA250.002097631836.018
ATGCCAT250.002097631836.06
ACAAGGA250.002097631836.017
CCCGACA250.002097631836.013
CGAGACG1450.034.13792823
CATTAAT1400.033.7532
ACGTTCA1500.033.00000427
AGACGTT1500.033.00000425
GAGACGT1500.033.00000424
GTTCATT1650.032.72727629
GTATGCC1450.032.58620542
ATGCCGT1450.032.58620544
CGTTCAT1600.032.3437528
TTCATTA1600.032.3437530
Produced by FastQC (version 0.11.2)