FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_11.351000000444ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_11.351000000444ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC32.7522935779816518RNA PCR Primer, Index 45 (95% over 21bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC21.834862385321101No Hit
TATCAACGCAGAGTACGGGAAGCAGTTGTATCAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCCCTAAAACCA10.9174311926605505No Hit
TCTTTATACAGATATCCACTCTTTGCCTCTTTTTACCCTTGTAAAGCTGTC10.9174311926605505No Hit
GGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTA10.9174311926605505No Hit
GAGTGAATAATAATGATGTGGGAGTGGGACTTATGTTGTTTTTTGTTTTTT10.9174311926605505No Hit
GTCACGTATGGTTCAACTCGGCGCAGCATGTTTACTGTTGCCTTGTTCACC10.9174311926605505No Hit
GAACAAGAGAAAGTAGACAGATACATGTTGGTAAATGCTAACTGTCCATAT10.9174311926605505No Hit
GAACAAAACCATAGTTGTGAGGGTACACAACTTATTAGAAGCTTTATCATA10.9174311926605505No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACT10.9174311926605505No Hit
CAACAAGAGTAGCGCATGATGCTTCAGCATATGAACCAAAGAGATCAGAAC10.9174311926605505No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10.9174311926605505No Hit
AAAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCAC10.9174311926605505No Hit
GGCGTGTCGCGCGTGGCGACTTGACGCACGGTCCAGCATCTGTCTCTTATA10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGTAACCAAT10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
CCTTGGAAGACACCATTAATTTAATCTCATTAACAGAATGAGAATAAATGA10.9174311926605505No Hit
CATGAGTATGGGAAAGAAACCTAGCAAGCTCACACAGATCATAGAATCTGT10.9174311926605505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTCCCCCCAGGCCGAACC10.9174311926605505No Hit
CTACTACACCGAGCACGACATGGGCGGCGAGAAGAAGCCTGTCGCCAGGAC10.9174311926605505No Hit
GCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAG10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCAATAAACACGTATGAAGAC10.9174311926605505No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCTGTGGCTTCTAC10.9174311926605505No Hit
ATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
ATCTCTGTCTATCTCTCTTGCGAAAAAAAGTACTGTTACTGTTGTTTGGTC10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAAACCAGC10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
CAACCGTCATTTTCCCCGAGTCCACAATCACTGGGTTGAATATCACTGGAT10.9174311926605505No Hit
GGTCCTAAGGTAGCGAAATTCCTCGTCGGGTAAGTTCCGACCTGCACGATT10.9174311926605505No Hit
CATTTAAGGACAACAATTCTGCCAGCAGAAGAAGAAATGCCAGAGATTATG10.9174311926605505No Hit
CTGCTACCATTGTGGGCGTATGTGTCCTTGAGCAAGACACTTAACGTCAAT10.9174311926605505No Hit
CTAACAAACTCTCAGAAAATGGAACTTTAACACTGTCTCTTATACACATCT10.9174311926605505No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTCATCATCC10.9174311926605505Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTTTTAAGCG10.9174311926605505Illumina PCR Primer Index 8 (96% over 27bp)
GTACGGGGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGA10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCACTTATCTCGTATGCCGTC10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAGTACTTTTTCTGTCT10.9174311926605505No Hit
GCATTTTTAGGGATTTCCTCAAGAACATCAAGAATCTTTGTGCTGTCTCTT10.9174311926605505No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACACAT10.9174311926605505No Hit
GGTATCAACGCAGAGTACGGGGCCTGTCTCTTATACACATCTCCGAGCCCA10.9174311926605505No Hit
TGTAAGCATCATCCTAGAAATAGCAACAAATCCCAAAATCACGAAACAGTA10.9174311926605505No Hit
GATAACACTCTCTGTGATGCTTAGGTGTAAGCCTGTCTCTTATACACATCT10.9174311926605505No Hit
TATCAACGCAGAGTACGGGACAGTGGTATCAACCTGTCTCTTATACACATC10.9174311926605505No Hit
GGGAAATCAACCGAGATTCCCCCAGTAGCGACGAGCGAACGGGGAGCAGCC10.9174311926605505No Hit
CTTTTTTTTTTTTTTTTTGCGTTGATACCACCCTTTTTCTTATAAACATCT10.9174311926605505No Hit
TTACAAACTTTTTTTACATCTATCACATGCACATCATATCCTTTTCCTGTC10.9174311926605505No Hit
CTGTGGTGGTTCGGGTCGTACCGGGCGAGACCGTGGTGGTAGAAGTCGTTT10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTATACCTGTATGGCGTT10.9174311926605505No Hit
ACTTTTATTCGATCGAATCATTTTCATTATTGCCATCACACATTCACAAGA10.9174311926605505No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG10.9174311926605505No Hit
GAGATGGACGATCAGGTTATGAGGCTTATCTAGTTAGTCACAACCTCCTCA10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTATCCCCACA10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
GCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGC10.9174311926605505No Hit
CATTGAGGTATAGCATTTACCAATATGCATTGTGCAGAGTATTAATCATAT10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACTCACCCT10.9174311926605505Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTT10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA10.9174311926605505No Hit
TCATGAGATGGTTCAAGTACAGTGTGTACAACCTGTCTCTTATACACATCA10.9174311926605505No Hit
GTATAATTATCTCATTCCTCGGATATATCTCTCCTTCTGCGGCGGCGACAA10.9174311926605505No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC10.9174311926605505No Hit
TGCTGAAATAACAAAATCTTTGCAAAGAGTTTTACACATGTCTCTTATACA10.9174311926605505No Hit
TTCTTCTTGTGTTTCCTCTTCTCCTATTCTGGAGAGGGATGAAGGAGATCC10.9174311926605505No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGACTCCTCTCC10.9174311926605505RNA PCR Primer, Index 36 (96% over 29bp)
GTATAAACTGAGAAATTGGAATTTGTGTAAATAAAAAATCTCCCCAAACAC10.9174311926605505No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAATACCCTTGTTTTAAACCTTTCCTT10.9174311926605505No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCTG10.9174311926605505No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10.9174311926605505No Hit
GAATTTTCTGTATGTAGACAACAGATGGCAAAATGGCTTCAAGCTTGGGTT10.9174311926605505No Hit
AACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGGAGCAGGG10.9174311926605505No Hit
CATAACAATAGAATCCCCAAAGAGTAAACATAACCATGAACGAACAATTAA10.9174311926605505No Hit
GAATGGATCAATGAGGATAACCTCAAAAAATTTGTATGTGGAATCTTCACC10.9174311926605505No Hit
GTAATACAATGTGCCAATCCATAAAATGTAAGTTAACAGGTTTCTCAATTT10.9174311926605505No Hit
ACGGGGAACAGACGCTTGTGGGGTGCACGCCAGCCGCTGGCAGCCATGGAC10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT10.9174311926605505No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT10.9174311926605505No Hit
CAGATGTTGAACGATCATAGTGGTGCCACGTATAATGTTAATGCTGTCTCT10.9174311926605505No Hit
CTTACAAGCAGTTAGAGTTCTATAGAATAGAATGATAACGTACCTTTTGCC10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGG10.9174311926605505No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG10.9174311926605505No Hit
GTGCAAATATTGATGCAATAGAAACTGCAAACACAATAGGAATAAGTGCTG10.9174311926605505No Hit
GACCATGTAAGTTCTTGGAGGTGTAATTTCTTCTTATTGGACTGTCTCTTA10.9174311926605505No Hit
GAACAATAGCACTCTTGACAAGTGTCTTAGTACGTACAAGCTCATTGTTGG10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCACTAATCTCGTATGCCGTC10.9174311926605505No Hit
GATTCTATCTCTTTTCTCAACTTTTCAAGAATCTCGGGATGGTTAATGAGC10.9174311926605505No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCT10.9174311926605505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAACCCCCCCCCC10.9174311926605505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9174311926605505No Hit
CTCCCACTGTCGACATTCTCTTAGACTACGATTGAAATGCTCTGTCTCTTA10.9174311926605505No Hit
ATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10.9174311926605505No Hit
GAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAA10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCCTGTCTCTTATACACATCTCCG10.9174311926605505No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA10.9174311926605505No Hit
TATCAACGCAGAGTACGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCC10.9174311926605505No Hit
ATGGATTCAGTTAATGATAGTGTGTCGAAACACCAGACTCTTATAAACATC10.9174311926605505No Hit
GAGTAGGAACCCATCCACGGAAAAAAGACTTTGACTCCTTGAACTTTCAGC10.9174311926605505No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAG10.9174311926605505No Hit
GAGCACCGCACGGCGGGTGTTATCGCAGCGTTATTGAGTCCATCGCCTCCT10.9174311926605505No Hit
GATTTGCGACAATTCTTTGATAATGATCCCTTCGTATGCCGTCTTCTGCTT10.9174311926605505Illumina Single End Adapter 2 (95% over 21bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTCACACTCTAT10.9174311926605505RNA PCR Primer, Index 36 (96% over 29bp)
CCACCTGCTCCGTTTAATGCCTTCACTCTCTCGATCAACTTGTTCTTCCTC10.9174311926605505No Hit
GTACTCTGCGTTGATACCATTGCTTCCCGTACCTGTCTCTTATACACATCC10.9174311926605505No Hit
CTCTGCCTTCTCTGATCCATTCAACACGTGCAATTTCCTGTCTCTTATACA10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATGCAGAC10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG10.9174311926605505No Hit
CCACGCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC10.9174311926605505No Hit
GCTCCCGAGAGAGCCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACC10.9174311926605505No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers