H23LNBCXX_l01n01_bir_0113_pb_10.35100000044475.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pb_10.35100000044475.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39651
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	13223	33.34846536026834	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT	1686	4.252099568737233	No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC	379	0.9558397013946686	RNA PCR Primer, Index 45 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG	317	0.7994754230662531	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	229	0.5775390280194699	No Hit
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT	95	0.2395904264709591	RNA PCR Primer, Index 45 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG	51	0.12862222894756753	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	42	0.1059241885450556	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	50	2.0008883E-11	45.0	45
TGCCGTC	1615	0.0	42.631577	45
ATGCCGT	1815	0.0	37.933884	44
TATGCCG	1860	0.0	37.01613	43
CTCGTAT	1865	0.0	36.91689	39
CCCACGA	1890	0.0	36.904762	19
ACGAGAC	1890	0.0	36.904762	22
GTATGCC	1860	0.0	36.89516	42
TCTCGTA	1860	0.0	36.89516	38
CGTATGC	1855	0.0	36.873314	41
TCGTATG	1870	0.0	36.818184	40
GCCCACG	1895	0.0	36.80739	18
CCACGAG	1895	0.0	36.80739	20
CACGAGA	1895	0.0	36.80739	21
ATCTCGT	1865	0.0	36.796246	37
CGAGACG	1890	0.0	36.785717	23
TCATTAA	1880	0.0	36.74202	31
AGACGTT	1875	0.0	36.719997	25
GAGACGT	1875	0.0	36.719997	24
CGTTCAT	1870	0.0	36.697865	28