FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pb_1.35100000044369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pb_1.35100000044369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34743
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC808523.27087470857439No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT7752.2306651699622946RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG2300.6620038568920358No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1820.5238465302363067RNA PCR Primer, Index 44 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC860.24753187692484815RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT400.11513110554644101RNA PCR Primer, Index 37 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9150.040.8196745
ATGCCGT10050.037.16417744
GCCGTCT751.8189894E-1236.00000445
TATCTCG10600.035.23584736
ACCCTAG10600.035.23584727
GAATATC10600.035.23584733
GACCCTA10600.035.23584726
AGACCCT10600.035.23584725
ATATCTC10600.035.23584735
CCTAGAA10600.035.23584729
CCCTAGA10600.035.23584728
GAGACCC10600.035.23584724
TAGAATA10600.035.23584731
GCCCACG10550.035.18957518
TATGCCG10550.035.18957543
CACGAGA10550.035.18957521
ACGAGAC10550.035.18957522
CTAGAAT10650.035.07042330
TCCGAGC10650.035.07042313
CTCCGAG10650.035.07042312
Produced by FastQC (version 0.11.2)