FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_96.35100000044340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_96.35100000044340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC2528.735632183908045No Hit
ATATGGAGCTTTTCATGTAATTACTCTTTTACTGCTGTCTCTTATACACAT11.1494252873563218No Hit
CAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGCAG11.1494252873563218No Hit
ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT11.1494252873563218No Hit
TCAGGGGTGTGGTTCTAAGCTGCATCAGTGGAACAAATACAAAATGCTGTC11.1494252873563218No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAACTGTCT11.1494252873563218No Hit
AACTGAAACATCTAAGTACCCCGAGGAAAAAAAAAAAAAAAAAAAAAAAAA11.1494252873563218No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGCTGTCTCTTATACACA11.1494252873563218No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCG11.1494252873563218No Hit
CGCCAGAAGTCGGCGAAGGCCGCGAACGGCACCTCCGGCTTGCCGATGTTC11.1494252873563218No Hit
GATTAGTAGTAGTTACTGGTTGAACATTGTTTGTTGGTGTATATCTGTCTC11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCATATGCCGTC11.1494252873563218No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG11.1494252873563218No Hit
AGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGT11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAGCATATCTCGTATGCCGTC11.1494252873563218No Hit
AGATCAGACAGTATGACCTGTTAAGCAAGCATTTTGAAATAATCAACTAAA11.1494252873563218No Hit
ACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTCTG11.1494252873563218Illumina PCR Primer Index 7 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT11.1494252873563218No Hit
TCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCCGTTACCCCACCTACT11.1494252873563218No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA11.1494252873563218No Hit
GAAACATCTAAGTACCCCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAACC11.1494252873563218No Hit
GTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA11.1494252873563218No Hit
GTTGTTTTCTTGACTTTGTTTTAGAATGAATCTCTTCTGCATCATCTAATA11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGCTTGCTGGAATCTCGTATGCCGTCT11.1494252873563218RNA PCR Primer, Index 8 (95% over 21bp)
TTGTTGGATAACTTTGAATGGCAAGATGGTTATAATAACAGATTCGGACTC11.1494252873563218No Hit
GTATATGAACCTTGTATTAGATGATGCAGAAGAGATTCATTCTAAAACAAA11.1494252873563218No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1494252873563218No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT11.1494252873563218No Hit
CTCTTATACACATCTCCGAGCCCACGCGACCCTGGATAATCTCGTATGCCG11.1494252873563218No Hit
AAAGTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGACTGTCTCTT11.1494252873563218No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC11.1494252873563218No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTC11.1494252873563218No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC11.1494252873563218No Hit
GGTTTAAGATGGATAAATGAATATCACTACTTGTACTAAAAACATGTTTGT11.1494252873563218No Hit
TCCTGGTGGTCAGGGCGCCATGGCGCTGTCCTGGCTGCAGCGCGTCGAGCT11.1494252873563218No Hit
GTTCAATATTCAGGAGATCTTACAACGTATGAAACCATTGCTTCAGCAAGA11.1494252873563218No Hit
AATTTCATATTTTTCAGATACTTCAGGAACACCTTCAGCTTCCAACTTCAC11.1494252873563218No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA11.1494252873563218No Hit
TCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCCTGT11.1494252873563218No Hit
GCCTTGCAGTTCAAGAACGTCTACCCCGAAAACTTCAAGGCCTGTCTCTTA11.1494252873563218No Hit
GGCCTTGGCTCAGGGCATGAAAATGCCCGTCTCTGTCTCTTATACACATCT11.1494252873563218No Hit
GGCGCGGACAAGGGTAGCGACGCGCCGCACGATCTGTTCGGCCTGATGGTC11.1494252873563218No Hit
GAAATATTCAGGCAAAAGGTATGTTAAACTCAATACTAGCTGCATGTTACC11.1494252873563218No Hit
CCGTTTACCGGGGCTTCGATCAAGAGCTTCGCTTGCGCCTGTCTCTTATAC11.1494252873563218No Hit
AAAGTACTCTGCGTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATC11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTTAATCTCGTATGCCGTC11.1494252873563218No Hit
CTATTGAAGCGCTACGTAGAGCTAAGTTTAAGTTTCCTGGGCGTCAGAAGA11.1494252873563218No Hit
GTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCCTGTCTCTTATACA11.1494252873563218No Hit
ATATAAGTGAGAACATGCAATATTTGTCCTTATGTGCCTTACTTATTTCAG11.1494252873563218No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA11.1494252873563218No Hit
GGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCA11.1494252873563218No Hit
ATACGGGACCCGCTATGGGGCCTCCCTCCGGAAAATGGTGAAGAAAATTGA11.1494252873563218No Hit
CCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATG11.1494252873563218No Hit
CATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG11.1494252873563218No Hit
GGGTGGCATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCT11.1494252873563218No Hit
CATCATTACATTCACAATTTACTCGAAAATTCGAACTGAAATTTTCCAATG11.1494252873563218No Hit
ATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAA11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACAGACGACGCTATATCTCGTATGCCGTCT11.1494252873563218RNA PCR Primer, Index 11 (95% over 21bp)
CAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGCCGTCCCTT11.1494252873563218No Hit
CTGACTGCCAGGGCATCCACCGTGTACGCTTAGTCGCCTGTCTCTTATACA11.1494252873563218No Hit
AAATTGGGAAAGGAGTAGAAAAAGCAGTAACTGACAACTTGAATAATACAC11.1494252873563218No Hit
AATACAATGACCACAATATTTTTTCAGGAAGAAAGATCAGCTCTCATTCTG11.1494252873563218No Hit
GTGATGCTGGGGAAAGGGAAGAGAGTGGCTCAGCCTGCAGGTAAATAGGCT11.1494252873563218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTG50.045.015
TGCATTT50.045.010
GTGCATT50.045.09
GTCACCC50.045.031
CGGGGCT50.045.024
CCTGTAT50.045.036
CACAATA50.045.01
GTGTACG50.045.019
TATGTGC50.045.06
TACGGGG50.045.022
TATCGCG50.045.040
TCGCGCG50.045.042
TTTTTGT50.045.014
GTATCGC50.045.039
GGCTGTC50.045.027
CATTTTT50.045.012
GCGCGCC50.045.044
TGTCACC50.045.030
CAATATG50.045.03
ATGTGCA50.045.07