FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_95.35100000044319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_95.35100000044319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21201
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC371417.518041601811234No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT4792.2593273902174427No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1140.5377104853544644No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG260.12263572472996558No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT230.10848544879958494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4300.043.4302345
CCACGAG4900.038.5714320
GATAATC4900.038.5714333
CACGAGA4900.038.5714321
CCCACGA4900.038.5714319
CTGGATA4900.038.5714330
TGGATAA4900.038.5714331
GACCCTG4900.038.5714326
AGACCCT4900.038.5714325
CCTGGAT4900.038.5714329
GTATGCC4850.038.5051542
AATCTCG4850.038.5051536
ATAATCT4850.038.5051534
TCTCGTA4850.038.5051538
ATGCCGT4850.038.5051544
CGTATGC4850.038.5051541
TCGTATG4800.038.43750440
CTCGTAT4800.038.43750439
GCCCACG4950.038.18181618
GAGCCCA4950.038.18181616