FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_93.351000000442d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_93.351000000442d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670902
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC64440.9604979564824669No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC15110.22521918253336554No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10300.1535246578486873No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT9710.1447305269622091No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA7970.11879529350039202No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7630.11372748926072661No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6840.10195229705679816No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC6790.1012070317274356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG8350.035.56874518
CCCACGA8350.035.56874519
TCGTATG8450.035.41408540
ATGCCGT8550.035.26041444
TATGCCG8500.035.20314443
CGTATGC8650.034.59526441
CTCGTAT8800.034.2612539
GAGACCC8750.034.19988624
GTATGCC8750.034.1973442
CTGGATA8900.034.129130
CCTGGAT8900.033.6234829
CACGAGA8900.033.37067421
CGAGACC9050.032.56895423
GGATAAT9550.032.27737832
GTCTAGT352.8105677E-432.159531
AATCTCG9550.031.57057636
GATAATC9700.031.54628833
ATCTCGT9650.031.47657837
TGGATAA9900.031.36353331
TCTCGTA9650.031.2434238
Produced by FastQC (version 0.11.2)