FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_92.351000000442ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_92.351000000442ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55848
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC36006.446067898581866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26724.784414840280762No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC15532.780762068471566No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA9351.6741870792150122No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC8851.5846583584013751No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8651.5488468700759204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7631.3662082796161008No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC6571.1764073914911903No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT5330.9543761638733707No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG5270.9436327173757342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.8988683569689156No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC4980.8917060593038246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.87559088955737No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA3940.7054863200114596No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT3730.6678842572697321No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC3720.6660936828534594No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGG3550.6356539177768228No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA3500.6267010456954591No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG3480.6231198968629137No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC2930.5246383039679129No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA2720.4870362412261854No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT2690.48166451797736715No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG2480.4440624552356396No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC2380.4261567110729122No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC2380.4261567110729122No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT2270.406460392493912No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTT1990.3563243088382753No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT1980.3545337344220026No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.3527431600057298No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC1810.324093969345366No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCT1780.31872224609654776No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTAC1740.31155994843145685No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC1700.3043976507663658No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.282910757771093No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC1580.282910757771093No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGA1540.275748460106002No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1510.2703767368571838No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG1480.2650050136083656No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA1380.24709926944563818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.234565248531729No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCA1280.22919352528291076No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1230.22024065320154704No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC1090.19517261137372868No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG1080.19338203695745596No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.17905744162727402No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.17905744162727402No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA960.17189514396218308No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC950.17010456954591033No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC940.16831399512963757No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA910.16294227188081936No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAA900.16115169746454663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.15936112304827388No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAG870.15577997421572842No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGG860.15398939979945567No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC840.15040825096691018No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.14861767655063746No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC820.1468271021343647No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.14503652771809197No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTA810.14503652771809197No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.13966480446927373No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA780.13966480446927373No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA750.13429308122045552No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTA720.12892135797163728No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.12175906030654635No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.11996848589027359No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA660.11817791147400085No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA650.11638733705772812No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC650.11638733705772812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4450.041.9662945
ATGCCGT4650.039.6774244
GAGACCC4750.038.84210624
CGAGACC4700.038.77659623
AGACCCT4700.038.77659625
GCCCACG4850.038.50515718
GATAATC4850.038.50515733
TAATCTC4850.038.50515735
TATGCCG4800.038.43749643
CACGAGA4800.038.43749621
CCCACGA4800.038.43749619
GTATGCC4750.038.3684242
ATCTCGT4750.038.3684237
ACCCTGG4750.038.3684227
CCCTGGA4750.038.3684228
CGTATGC4750.038.3684241
GACCCTG4750.038.3684226
CCTGGAT4750.038.3684229
TCGTATG4700.038.2978740
CTCGTAT4700.038.2978739
Produced by FastQC (version 0.11.2)