FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_89.3510000004425e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_89.3510000004425e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC32618.88760139049826No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT241.3904982618771726No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG80.4634994206257242No Hit
CTTATACACATCTCCGAGCCCATGAGACCCTGGATAATCTCGTATGCCGTC70.4055619930475087No Hit
CTTATACAATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT70.4055619930475087No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC60.34762456546929316Illumina PCR Primer Index 7 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT60.34762456546929316No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG50.28968713789107764No Hit
CTTATACATATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC40.2317497103128621No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT40.2317497103128621No Hit
CTTATACGCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC40.2317497103128621No Hit
CTTGTACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC40.2317497103128621No Hit
CTTATACACACTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT40.2317497103128621No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT40.2317497103128621No Hit
CTTATACTCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT30.17381228273464658No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATTTCGTATGCCGTC30.17381228273464658No Hit
CTTATAAACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACACATCTCCGAGCCCCACGAGACCCTGGATAATCTCGTATGCCGT30.17381228273464658No Hit
CTTATAACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT30.17381228273464658No Hit
CTTATTACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT30.17381228273464658No Hit
CTTACACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACACAACTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
ATTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACACATCTCCGAACCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CTTATACAAATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.17381228273464658No Hit
CCTGCCAGGTGGCATGCGCCGCATCCTCGTTGCCGAGGAGGCTGGAGAGGA20.11587485515643105No Hit
CTTATACACGTCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACAAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
ATCGATATGATCGTGGTGGTGACCGACATGATAGATATTATGAACGTGACC20.11587485515643105No Hit
GGCTGAAGCTGCGGGACCGTCACCCGAACCTGCTCGACGAGATGCGCGCCA20.11587485515643105No Hit
GGGCTGGGCCAAGCCCGGCGGCGCGAACCAGGCGGTCACCGCAAAGACGCC20.11587485515643105No Hit
GCCGCGATCCTCCTGGGCCTGATCCCCTACACCTTCCAGGACCCGGCCAAC20.11587485515643105No Hit
ACAGGAGAGAGCTCACTTCTTCTCTGCTTGTAAGCACCAAGGAAAGACCAC20.11587485515643105No Hit
CTTATACACATCTCCGAGCACACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACGGGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
AACCGGGCGGTTGAACCAAATTGCGGGCTTTTGGCCAAAGCAGGATTGTAC20.11587485515643105No Hit
CTCGACGTCGTGGAGACCGGCCTCCGCGAGCTTGCGGTTCAGCACGGCCTC20.11587485515643105No Hit
CTCATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
CTCTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACGAGAACCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
AGATGAAGGCGCTGCAGGATGAACTCGACACCATCGAGGTCGAAGGCCTGT20.11587485515643105No Hit
AAATAATCCAATGAGTGAATGACCGACAAGTGCCCCACCAGAATACACAAG20.11587485515643105No Hit
GCGGCTTGTCGATCAGCAGCGTGGGGATTGCGCGGGGGGCGGAAGCCGCGT20.11587485515643105No Hit
GTGGTGTACGTCCCGGCGCCGGCGGATGACGGCGCCGAGAATGACGCGGGC20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGGC20.11587485515643105No Hit
CCTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
CTTATACACCTCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
GCTTGATGAACTCGGTCGGCGCGTCGGCGGGCACCTTGCGGGCGTTGTCGC20.11587485515643105No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT20.11587485515643105Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACCACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT20.11587485515643105No Hit
CATATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC20.11587485515643105No Hit
CTTATACACTCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT20.11587485515643105No Hit
GGTAGGTCTTGGACATGGGTCTCCTTGAGGGTGTTGGGTTGGCAGTGGTGG20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCCCGTATGCCGTC20.11587485515643105No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTAAGCCGTC20.11587485515643105No Hit
TGATTGCCGAAATTCTTGGAATCTATCCGTAAATTATATTTGCGCGTAGTG20.11587485515643105No Hit
CATGCGGGGTGGGGTGCGCTTTGAGCCAGGCCGCCCGAGGGTGTTCGGGCC20.11587485515643105No Hit
GGATAGAGGTGTGCGCAGGATTCGACTACCAAGGTGGGCCCATCACCGACA20.11587485515643105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC550.045.045
CGAGCCC650.041.5384615
CCGAGCC650.041.5384614
CTCCGAG650.041.5384612
AGCCCAC600.041.24999617
ATCTCCG600.041.24999610
GCCCACG600.041.24999618
TATACAC600.041.2499963
CATCTCC600.041.2499969
GAGCCCA600.041.24999616
CTTATAC600.041.2499961
TCTCCGA600.041.24999611
ATACACA600.041.2499964
CACATCT551.0913936E-1140.9090927
ACATCTC551.0913936E-1140.9090928
TTATACA551.0913936E-1140.9090922
TACACAT551.0913936E-1140.9090925
ACACATC551.0913936E-1140.9090926
TCCGAGC700.038.57142613
GTATGCC651.8189894E-1238.0769242
Produced by FastQC (version 0.11.2)