FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_88.35100000044234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_88.35100000044234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT45.063291139240507No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC33.79746835443038No Hit
ACATAACAGAGTTTTTGGCATTTTTTTTCAGCAAGTTGAATATCAGGTTGC11.2658227848101267No Hit
GGGTTTCACCACGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTAAT11.2658227848101267No Hit
CTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT11.2658227848101267RNA PCR Primer, Index 44 (95% over 22bp)
GTATTAGCAGCTTGGAAAGTTCCTGTGAAGAATGATACTTTCTTACCTGTC11.2658227848101267No Hit
GGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCT11.2658227848101267No Hit
ATTCTGTACAAGGTCTTCACTTTGCTGTCATCATTTGTACAAACTCTTCAT11.2658227848101267No Hit
GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAACTGTCTCTTATACAC11.2658227848101267No Hit
TATCAACGCAGAGTACGGGAAGCCTGTCTCTTATACACATCTCCGAGCCCA11.2658227848101267No Hit
GATGGATGTCTTTGCTGAAATCTAACAATTAGCTCATATTCCATGAGAAAG11.2658227848101267No Hit
GCTTTTATCTGCTAATAATTCTGCTACGTGTTTATGACCATTCTGAGATGC11.2658227848101267No Hit
GATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTG11.2658227848101267No Hit
TCTTATACACATCCCCGAGACCACGAGACAACATAATATCTCGTATGCCGT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.2658227848101267No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGCTG11.2658227848101267No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCGTACCTTT11.2658227848101267No Hit
GTTGTCAGGAGACCTCCCATTCATAGGAGAAGTTTCAGTCTAGAAGCTGTC11.2658227848101267No Hit
ACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCTGTCTCTTATA11.2658227848101267No Hit
CCAAAATATGCTAGAAACATCCTGAGATTCTGTGACTACTGTTCTGACATT11.2658227848101267No Hit
CTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTCTCTGCTGTCTCT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATTTCGTATGCCGTC11.2658227848101267No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTCTC11.2658227848101267No Hit
GTATCAACGCAGAGTACGGGGCCCAGAGCCTGAATCAGTGTGTGTGTTAGT11.2658227848101267No Hit
CTTATACACATCTCTGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATACTATCTCGTATGCCGTC11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGTAATATCTCGTATGCCGTC11.2658227848101267No Hit
GTCCACCACCACTACCACTGCCAGCACAGCTGTTGCAGATGCTCAGAAGAG11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCAGAGACGACGCTATATCTCGTATGCCGTCT11.2658227848101267Illumina PCR Primer Index 11 (95% over 21bp)
AAAATGCACACTCTGTAGTTCTGAATAATAAAAGGAAAATGGCACCAACTA11.2658227848101267No Hit
ACACAAAACTGAAACAAAAAGGCCATTTCTATAATACCTGTCTCTTATACA11.2658227848101267No Hit
TCTCACATCACTGTATGTATAAAGTTATGCCTTGCTCTCTGACTGTCTCTT11.2658227848101267No Hit
GTACGGGGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTA11.2658227848101267No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC11.2658227848101267No Hit
GAGGAAGTAGGATTTATCAAGTAGAAAAGGAAGTGTAAAGAAGGCAGAAGT11.2658227848101267No Hit
TATCAACGCAGAGTACGGGAGTACCTGTCTCTTATACACATCTCCGAGCCC11.2658227848101267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTGTAACCCCCCCACCCCT11.2658227848101267No Hit
CCGGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTCTGTC11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAACCTATCTCGTATGCCGTC11.2658227848101267No Hit
GCTGCAGACCGTGCCGATGATCATCAACGCCCGCACCCTGTTCGCGCGGCT11.2658227848101267No Hit
GCTATTGAAAAAATCAGACGAACAGCCATTTCTTTTATTCAAATTGAGTAT11.2658227848101267No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAGTCCTCCTGTCTCTTAAACCCTCCT11.2658227848101267No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT11.2658227848101267No Hit
GTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTG11.2658227848101267No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA11.2658227848101267No Hit
ATGTCCAGCTATGTTAACTAAATCAGTCTTTCACCTGTCTCTTATACACAT11.2658227848101267No Hit
GCCTATAGCTTATGTTAAATGGTTGAAATGGTTCTTTTTATAGCTGTCTCT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATCCTATCTCATATGCCGTC11.2658227848101267No Hit
ACCTTTCAATCCGTTCCAAAACAAATGGAAATTGATGGTAAAGATGGGATT11.2658227848101267No Hit
GCTAATTGTTAAACATTCATCAGCACATGATTTGCTGTCTCTTATACACAT11.2658227848101267No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTTATACAC11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACATTATAATATCTCGTATGCCGTC11.2658227848101267RNA PCR Primer, Index 44 (95% over 24bp)
CTGTAAGACCTAAAGTTCTTATGAGATTGTCCCTGTCTCTTATACACATCC11.2658227848101267No Hit
GATGTATTATGGGACCTTATTGGTAACAAACAGCAATGTTCCTGTCTCTGA11.2658227848101267No Hit
AGGTTAAACAAACATACCCTTTTGTACACAAAGGGGAACATACTATATACA11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACCACATGACATCTCGTATGCCGTC11.2658227848101267RNA PCR Primer, Index 34 (95% over 21bp)
ATGGAATGGAATGGAATGGAATGGAATGGAATTAACCCGAATAAAATGGAA11.2658227848101267No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACGACCTAATATCTCGTATGCCGTC11.2658227848101267No Hit
GTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATACAC11.2658227848101267No Hit
CTTATACACATTCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATACTATCTCGTATGCCGTC11.2658227848101267No Hit
GTATCAACGCAGAGTACGTGGTATCAACGCAGCGTACTTGTTCTGTCTCAT11.2658227848101267No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATGATATCTCGTATGCCGT11.2658227848101267No Hit
TTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGCTGTCTCTTATACACATCT11.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACAACAAACTATCTCGTATGCCGTC11.2658227848101267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTAAGTTGTTAA11.2658227848101267No Hit
CCCCGGACCAGGCAGCGCGCCGCTGCGGCCAGGACGCCCATGCCGCGCCCT11.2658227848101267No Hit
GGACAAAGGACATCTAAAAACAAACTGTATCCTTCCTGTCTCTTATACACA11.2658227848101267No Hit
AAACAGAATTCTATTTGGGCAAGAGATGCGCTTATGTATCTGTCTCTTATA11.2658227848101267No Hit
CATCCATTGGATCATTAAAAAAGCAAGAGAGTTCTAGAAAAACATCTACTT11.2658227848101267No Hit
GGCATAATTTGTTTTAGTTGATATTCAAGGCTTCAAAAGTCATTATTCCTG11.2658227848101267No Hit
ATTGATTACCTTCTTTGAATTGCATTGATTCTGTCTCTTATACACATCTCC11.2658227848101267No Hit
CGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAA11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
GTATGCC50.045.042
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
GCCCACG50.045.018
TATACAC50.045.03
TGCCGTC50.045.045
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCC50.045.09
CAACATA50.045.028
GAGCCCA50.045.016
CACGAGA50.045.021
CCCACGA50.045.019
ACATCTC50.045.08
TTATACA50.045.02
TACACAT50.045.05
ACACATC50.045.06
ATAATAT50.045.032
Produced by FastQC (version 0.11.2)