FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_85.351000000441e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_85.351000000441e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196729
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC9944550.549232700821946No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT3531217.949565137829197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG137626.995409929395259No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGC2040.10369594721672962No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG253.879243E-544.99949313
GAGTACG253.879243E-544.99949312
TGCCGTC105200.044.57174345
GCCGTCT900.039.9995545
AGAACAC250.00210299135.9995967
ATATGCC250.00210299135.99959642
ATGCCGT141400.033.1767844
GTATTTG352.8037437E-432.1588441
TATGCCG155400.030.18788343
GTATGCC155450.030.12027742
TATCTCG154500.030.10160336
ATCTCGT154750.030.0820537
TCTCGTA154850.030.07715238
GCCCACG156250.030.0668618
AATATCT154850.030.06262234
CACGAGA156450.030.05718621
CGAGACA156100.030.05251123
AGCCCAC156350.030.04763217
CCCACGA156350.030.04763219
TCCGAGC156350.030.04763213
Produced by FastQC (version 0.11.2)