FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_83.351000000441af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_83.351000000441af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231672
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC130685.6407334507407025No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT58492.5246900790773164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG16490.7117821747988535No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4410.1903553299492386No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4270.18431230360164372No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4070.1756794088193653No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3170.13683138229911254No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3090.1333782243862012No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3000.1294934217341759No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2910.12560861908215062No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2750.11870230325632791No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2670.11524914534341654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14800.044.23816345
TATAACG301.1359223E-437.5066532
ATGCCGT21000.031.17737444
TAACGCA702.8603608E-828.9337064
TATGCCG22850.028.65316643
GTATGCC23000.028.46629742
TATCTCG22950.028.4302836
CGTATGC23050.028.40455241
TCGTATG23050.028.30694240
ATAGAAC406.144786E-428.1299923
CTCGTAT23250.028.0634439
TCTCGTA23250.028.0634438
ATAATAT23500.027.95637332
TAATATC23700.027.81538633
CATAATA23700.027.81538631
CGAGACA23550.027.8014823
ATCTCGT23550.027.70594237
CAACATA23800.027.69851528
ACATAAT23850.027.6404530
ACGAGAC23700.027.6255222