FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_82.35100000044185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_82.35100000044185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295544
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC86122.9139485152802966No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT12880.4358065127358363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.19489483799366591No Hit
GGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCAT4430.14989307852637845No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4160.1407573829954254No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG4080.13805051024551335No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4050.13703543296429635No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.12959153290203826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.12553122377717021No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3550.12011747827734619No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC3550.12011747827734619No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3200.10827490999648105No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT3200.10827490999648105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.10793655090274205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10400.040.45931645
ATGCCGT11550.036.430944
TATGCCG12100.034.77495243
GTATGCC12200.034.67434742
TCTCGTA12200.034.66848438
CATAATA12300.034.57538231
AATATCT12250.034.53281834
CTCGTAT12250.034.52697839
ATATCTC12200.034.4899135
ATCTCGT12200.034.48407437
CGTATGC12200.034.48407441
TATCTCG12200.034.48407436
CAACATA12400.034.4780128
ACATAAT12500.034.382230
TCGTATG12250.034.34332340
TAATATC12350.034.25320433
ATAATAT12350.034.25320432
AACATAA12700.034.195129
GACAACA12600.034.1093226
CGAGACA12600.033.9307423
Produced by FastQC (version 0.11.2)