FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_8.3510000004384e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_8.3510000004384e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC242.0618556701030926RNA PCR Primer, Index 27 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG70.6013745704467355No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT40.3436426116838488No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC40.3436426116838488No Hit
GTATACGCTTGTAACTGTTTGATTACATTTTTCTACTTAGCCAATCAATCA30.25773195876288657No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC30.25773195876288657No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC20.1718213058419244No Hit
ATTATGGCAAAAAAGAAAACAAAATTCTATGCAGTTCGTAATACTGTCTCT20.1718213058419244No Hit
CAATTCGTTGTATTTACAACCTTGTGATCTCACATACACAGAATCATTTAC20.1718213058419244No Hit
CATAGTCACTCTCCGACTCCAGGTCATCATCTGATAAGTCAGAATGCTGTC20.1718213058419244No Hit
CTTTACAATAACCTGCCCTAATTGTAACCATCAATTTGAACCCGGTGAAAC20.1718213058419244No Hit
TTTTAGTCCTTGGTAAATACCTAGAATTGGGAGTACATGTCCTTATGTGAA20.1718213058419244No Hit
GAATCGCGTCGATGCCCGACGTGCCGGAGACAATCACGATCGGTGCGCGGG20.1718213058419244No Hit
GAGCATACCATTGCAAAAAATGTGTAAAGAAGAAGAAATGGGAGGACCAAA20.1718213058419244No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC20.1718213058419244No Hit
CATTGGGGCCGACCTGGTTTCGACGTGGGTTACAAAGCAGTGGAGGGCATA20.1718213058419244No Hit
GTGATAATTTTAAATTCATTCCCATCATTCCCCAAACCATAATTGGCACAC20.1718213058419244No Hit
CTCCTTGATCATTTAAAAAAGTACGAATGGTGTTTATAACTGTCTCTTATA20.1718213058419244No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)