FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_79.3510000004411b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_79.3510000004411b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70106
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC18822.684506319002653No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC2800.3993952015519356No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT2290.3266482184121188No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT1860.2653125267452144No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG1730.24676917810173166No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCG1570.22394659515590676No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGAT1530.21824094941945055No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT1470.20968248081476623No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC1430.20397683507831No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT1420.20255042364419593No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTC1280.18258066356659916No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCC1210.17259578352780075No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAA1190.16974296065957264No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG1130.1611844920548883No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTG1120.15975808062077426No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1120.15975808062077426No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTT1120.15975808062077426No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC1090.1554788463184321No Hit
CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGG1090.1554788463184321No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT1070.15262602345020398No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGAT1060.15119961201608992No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGG1050.14977320058197585No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC1010.14406755484551964No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGC1000.1426411434114056No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTG990.14121473197729154No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAG960.13693549767494936No Hit
GGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTCGAAAGGGGATCCGG940.13408267480672126No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTC940.13408267480672126No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA910.1298034405043791No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGC900.12837702907026505No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGC900.12837702907026505No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGGA900.12837702907026505No Hit
GGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGC890.12695061763615098No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA870.12409779476792286No Hit
GGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTA870.12409779476792286No Hit
GCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCG840.1198185604655807No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC830.11839214903146664No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGA800.11411291472912446No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA800.11411291472912446No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCG800.11411291472912446No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT790.11268650329501041No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCG770.1098336804267823No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA760.10840726899266824No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGA750.10698085755855419No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTT730.10412803469032608No Hit
GAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGATTACG730.10412803469032608No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG720.10270162325621202No Hit
GGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAA720.10270162325621202No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA710.10127521182209796No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATG710.10127521182209796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGAT206.9890934E-444.99857323
ATGCCGT2000.037.12382544
GCGACCT504.7693902E-835.9988613
CGCGCGA504.7693902E-835.9988610
CCCACGA2250.034.9988919
ACTTGCT2350.034.4669927
CTTGCTG2350.034.4669928
AGACTTG2350.034.4669925
GACTTGC2350.034.4669926
GCCCACG2300.034.23804518
CACGAGA2300.034.23804521
ACGAGAC2300.034.23804522
CGTATGC2300.034.23804541
CGAGACT2300.034.23804523
CTCGTAT2300.034.23804539
GAGACTT2300.034.23804524
TTGCTGG2300.034.23804529
GTATGCC2250.033.9989242
TATGCCG2250.033.9989243
TCGTATG2250.033.9989240
Produced by FastQC (version 0.11.2)