FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_78.351000000440f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_78.351000000440f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419640
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC5870813.990086741016109No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT235655.615527595081499No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG27450.6541321132399199No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC23690.5645315031932132No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA19100.45515203507768565No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC19060.45419883709846537No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA16370.39009627299590127No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA14610.34815556191020874No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT13270.3162234296063292No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC11920.28405299780764465No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA11070.26379754074921363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA10530.2509293680297398No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA10100.24068248975312173No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC9760.23258030692974932No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT9660.2301973119816986No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8630.20565246401677625No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC8340.1987417786674292No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC8250.1965970832141836No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT7660.1825374130206844No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC7520.1792012200934134No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6910.1646649509103041No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT6450.15370317414927082No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA6360.15155847869602518No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT6350.1513201792012201No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG5820.13869030597655133No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA5760.1372605090077209No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5710.13606901153369555No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT5670.13511581355447527No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT5650.1346392145648651No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC5570.13273281860642455No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5560.13249451911161947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG5540.13201792012200933No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5520.1315413211323992No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC5480.13058812315317891No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC5370.1279668287103231No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5320.12677533123629778No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA5220.12439233628824707No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4890.11652845295967973No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC4780.11390715851682394No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG4730.11271566104279859No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC4640.11057096558955296No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4640.11057096558955296No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT4500.10723477266228196No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA4490.10699647316747687No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA4390.10461347821942618No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC4390.10461347821942618No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4330.10318368125059574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT207.031922E-444.9951138
TGCCGTC62600.044.24040645
GCCGTCT1050.036.42461845
TTAGATA250.002104246536.0046733
TATAGAG250.002104246536.0046733
ATGCCGT88150.031.41746144
GCTGGAA91250.030.2587231
TATGCCG91400.030.25109143
ACGAGAC91950.030.24501822
CGAGACT91900.030.23699223
CTCGTAT90800.030.22799339
AGACTTG91750.030.21285225
GACTTGC91700.030.20839126
ATCTCGT90800.030.20321737
CACGAGA91950.030.1960821
ACTTGCT91550.030.18414727
GAGACTT92150.030.17937724
TCGTATG90800.030.1784440
GTATGCC91400.030.17724842
TGCTGGA91500.030.17604430
Produced by FastQC (version 0.11.2)