FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_77.351000000440d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_77.351000000440d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307890
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC9075629.476761180941246No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT4011313.028354282373575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG54681.775958946376953No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACA11130.3614927409139628No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10080.3273896521484946No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA8640.28061970184156676No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC7610.24716619571925036No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAG6490.21078956770275098No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGG6350.20624248920068858No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6290.20429374127123326No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5910.1919516710513495No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5400.17538731365097923No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGAT5170.16791711325473382No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATG4590.14907921660333234No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4500.14615609470914936No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4460.14485692942284584No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4450.14453213810126994No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4200.13641235506187274No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA4170.13543798109714508No Hit
AAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAG4030.13089090259508265No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3950.12829257202247557No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3800.12342070219883725No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3790.12309591087726135No Hit
TGTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAA3790.12309591087726135No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTC3660.11887362369677483No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3470.11270258858683296No Hit
CTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCC3400.11042904933580173No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAG3310.10750592744161877No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGA3300.10718113612004287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAG207.0262287E-444.99870338
TGCTGCA207.0262287E-444.99870337
CTGCAGA207.0262287E-444.99870339
GTGCTGC207.0318545E-444.99139436
AGCTGTA207.0318545E-444.9913947
ACGCTTA207.0318545E-444.99139414
GTAACGC207.0318545E-444.99139411
TGCCGTC95400.044.5034345
TTGATGG250.002103337636.0048129
GCAGATA250.002103337636.0048141
GAATCGC250.002105015635.99896235
CGCGTAT250.002105015635.99896239
TGTAACG250.002106694735.99311410
AATCGCG250.002106694735.99311436
CGCTTAA250.002106694735.99311415
GACTTTC653.6561687E-1034.62000726
GCCGTCT1000.033.74902745
AGACTTT708.076313E-1032.14715225
CATAGGC352.813405E-432.14715225
ATTGAGT352.816089E-432.141934
Produced by FastQC (version 0.11.2)