FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_75.35100000044093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_75.35100000044093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244166
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC188517.720567155132165No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT52012.1301082050735975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG9430.38621265860111564No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC9350.38293619914320587No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC6390.2617071992005439No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA6280.25720206744591795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6060.24819180393666607No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4470.18307217221070912No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3330.13638262493549472No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2960.12122899994266197No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2960.12122899994266197No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC2880.11795254048475216No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC2770.11344740873012622No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2620.10730404724654538No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2490.101979800627442No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG2450.1003415708984871No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGAC207.0236216E-444.99836331
GGGTAGC207.0236216E-444.99836330
TATTCGG207.0236216E-444.99836336
TTCGGAG207.0236216E-444.99836338
ATATTCG207.0236216E-444.99836335
TGCCGTC19800.043.4075145
TACCCAG301.136163E-437.5063135
GGGACTA301.1375342E-437.49863435
GCTTGCA250.002102127836.006062
TGAGGGT250.002102127836.006064
ATTCGGA250.002104242635.99868837
AAGCGTC250.002104242635.99868820
GCCATAG250.002104242635.99868835
GTCAGGG250.002104242635.99868821
ATATCGA250.002104242635.99868824
TCGGAGA250.002104242635.99868839
GTTAAGG250.002104242635.99868822
GGGGTTT250.002104242635.99868839
GAGCAGG250.002104242635.99868842
AGGGATT250.002104242635.99868824
Produced by FastQC (version 0.11.2)