FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_73.35100000044050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_73.35100000044050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464596
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC24320.5234655485626222No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG6460.1390455363369465No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC5720.12311771948101147No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4970.10697466185675296No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4750.10223936495363714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3300.036.13567443
GTATGCC3300.036.13567442
ATGCCGT3300.036.13567444
CTAGGTC250.002106016735.9993134
CTCGTAT3500.034.7136239
CGTATGC3450.034.56455641
TCGTATG3500.034.07077440
CGAGACT3800.031.38097823
TCTCGTA4000.030.3744238
CGACCGT300.005144619429.9994266
TCGGCCC300.005144619429.99942615
GACTTGC4000.029.8119326
GCCCACG3950.029.61968418
CCCACGA4050.029.4438819
ATCTCGT4150.029.27654837
ACTTGCT4150.029.27654827
AATCTCG4200.028.92801736
CACGAGA4400.027.10175321
TTGCTGG4600.026.41253729
ATGTAAG608.124498E-626.272121
Produced by FastQC (version 0.11.2)