FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_72.35100000044036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_72.35100000044036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173384
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC2233512.88181147049324No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT94615.456674202925299No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG12720.7336317076546855No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGC207.0198067E-444.995968
AGCCCTC406.7630026E-944.9959616
TGCCGTC24800.043.36304545
CTCCAAC250.002097162236.0175481
CCTAGAC250.00210311335.996773
CAGCCCT504.841968E-835.9967715
TCAGCCC504.841968E-835.9967714
TTGGGCA250.00210311335.9967716
AGTCTCA606.370101E-933.74697510
GGAAGTT352.8120514E-432.13997338
ACTACGA352.8120514E-432.1399738
AAGTCTC651.3902536E-831.1510499
ATGCCGT35000.030.72581544
GATAAGT300.005130356630.00596435
GTTATGT602.3947905E-730.00596434
GAGAGGA300.00513760229.997317
ATATCGT300.00513760229.997316
AGTCTTA300.00513760229.997318
TGGGCAA300.00513760229.9973117
CTCAGCC602.400866E-729.9973113
Produced by FastQC (version 0.11.2)