H23LNBCXX_l01n01_bir_0113_pa_71.35100000044029.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0113_pa_71.35100000044029.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141810
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	13922	9.817361258021295	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	2468	1.740356815457302	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	797	0.562019603695085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	567	0.3998307594668923	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG	515	0.3631619772935618	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	484	0.3413017417671532	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	436	0.30745363514561735	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	428	0.30181228404202803	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	427	0.3011071151540794	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC	367	0.2587969818771596	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT	342	0.24116775967844298	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	336	0.236936746350751	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.23059022635921303	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC	311	0.21930752415203442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	309	0.21789718637613711	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC	295	0.2080248219448558	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	270	0.1903955997461392	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA	269	0.18969043085819054	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	247	0.17417671532331994	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	246	0.17347154643537127	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	245	0.1727663775474226	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	239	0.16853536421973064	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	221	0.15584232423665467	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA	197	0.13891827092588674	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	196	0.1382131020379381	No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC	196	0.1382131020379381	No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	195	0.1375079331499894	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	193	0.1360975953740921	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	182	0.1283407376066568	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC	170	0.11987871095127282	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159	0.11212185318383752	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	158	0.11141668429588887	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	157	0.1107115154079402	No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC	157	0.1107115154079402	No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC	156	0.11000634651999154	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	155	0.10930117763204288	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	154	0.1085960087440942	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA	153	0.10789083985614555	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	146	0.1029546576405049	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	146	0.1029546576405049	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTCTT	20	6.993953E-4	45.024	44
GTATAGA	20	7.006128E-4	45.008114	1
AATCCCT	20	7.018318E-4	44.992245	11
TGCCGTC	1485	0.0	44.417614	45
ATGCCGT	1720	0.0	38.47981	44
TATGCCG	1745	0.0	37.786182	43
TCTCGTA	1735	0.0	37.601585	38
TCGTATG	1735	0.0	37.601585	40
GTATGCC	1750	0.0	37.549625	42
CTCGTAT	1740	0.0	37.493534	39
TATCTCG	1740	0.0	37.493534	36
CGCTATA	1760	0.0	37.464138	31
ACGCTAT	1760	0.0	37.464138	30
GACGCTA	1760	0.0	37.464138	29
GACGACG	1755	0.0	37.442646	26
ACGAGAC	1755	0.0	37.442646	22
CGTATGC	1750	0.0	37.407837	41
CGACGCT	1765	0.0	37.358006	28
ACGACGC	1765	0.0	37.358006	27
AGACGAC	1760	0.0	37.336277	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content