FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_70.3510000004400f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_70.3510000004400f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294380
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC208507.0826822474352875No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT91123.0953189754738775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG9830.33392214144982674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22550.043.40354545
GCCGATC250.00210456836.00000420
CTATGCA352.815315E-432.1428579
GCCGTCT801.0004442E-1030.937545
ATGCCGT32150.030.44323544
TACCCGC300.005141111630.0000025
CGAGTTG300.005141111630.00000240
GTCGAAC300.005141111630.00000218
GGACCGA300.005141111630.00000239
ACCCGCT300.005141111630.0000026
CCCGCTG300.005141111630.0000027
TATCTCG33200.029.34488136
CTCGTAT33050.029.27382939
ATCTCGT33150.029.1855237
TATGCCG33400.029.16916343
CGACGCT33500.029.14925428
ACGACGC33500.029.14925427
CGCTATA33550.029.1058131
CGTATGC33500.029.0820941
GACGCTA33500.029.0820929
Produced by FastQC (version 0.11.2)