FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_69.35100000043fe7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_69.35100000043fe7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163558
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC11216168.57567346140208No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1938611.852676114895022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG68364.179557099010749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC207.0110656E-445.00581433
TATAACT207.0110656E-445.00581434
CGCCATA207.0110656E-445.00581431
GACGACC302.152141E-645.00580626
ACGACCC302.152141E-645.00580627
ACTCGTA207.02164E-444.99205438
AACTCGT207.02164E-444.99205437
TAACTCG207.02164E-444.99205436
TGCCGTC114550.044.67783445
GCCGTCT800.042.1800545
ATGCCGT135450.037.7840244
CATATCT301.134782E-437.5048434
CGACCCT301.134782E-437.5048428
CCTATAT250.002100506336.0046532
ATAACTC250.002100506336.0046535
ACCCTAT250.002100506336.0046530
GACCCTA250.002100506336.0046529
CCCTATA250.002100506336.0046531
TCCGAGC144200.035.56930513
GCCCACG144450.035.53889518
Produced by FastQC (version 0.11.2)