FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_68.35100000043fcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_68.35100000043fcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100517
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC31653.1487211118517267No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT7950.7909109901807654No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2530.25169871762985363No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2460.24473472148989722No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1980.1969816051016246No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA1940.1930021787359352No Hit
GTTACAAAAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1760.17509476009033298No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA1500.14922848871335195No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG1440.1432593491648179No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1360.13530049643343914No Hit
CTATTACGTCGATTTCAAAAATAACCTCACACGTTATGAGAAAGAATCAGC1280.12734164370206033No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC1240.12336221733637097No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC1220.12137250415352628No Hit
TTGTAATACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA1210.12037764756210392No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG1180.11739307778783688No Hit
GTTATAATAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1070.10644965528219107No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT1060.10545479869076872No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG1060.10545479869076872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4000.041.63144745
CGGGGCA250.002096392736.00557317
ATGCCGT5050.032.97540344
TATCTCG5000.032.40501836
CTCGTAT5050.032.08417539
CGAGACG5050.032.08417523
TCGTATG5100.031.76962540
GTATGCC5250.031.71919642
CGTATGC5200.031.59142941
TGTAGTA501.9631807E-631.4892042
CACGAGA5150.031.4611821
TATGCCG5300.031.4199643
CGCTATA5100.031.3283831
GAGACGA5250.031.2905624
ACGACGC5250.031.2905627
CGACGCT5050.031.19294728
GACGCTA5050.031.19294729
ACGAGAC5200.031.1586722
AGACGAC5300.030.99536725
TATATCT5100.030.88713534
Produced by FastQC (version 0.11.2)