FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_66.35100000043f8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_66.35100000043f8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114418
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC31372.741701480536279No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT6430.5619745144994669No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2120.1852855319967138No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1440.12585432362040938No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA1370.11973640511108391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3650.040.06771545
ACCTATA301.12781134E-437.5320781
ATGCCGT3900.037.4992744
CTCGTAT3900.035.19162439
TCTCGTA4000.034.8743238
CGCTATA4100.034.572531
ACGACGC4100.034.572527
CGACGCT4050.034.44377528
GACGCTA4050.034.44377529
TATGCCG4250.034.411143
GTATGCC4250.034.411142
GCTATAT4150.034.15596432
GACGACG4150.034.15596426
TATATCT4100.034.02372734
ACGCTAT4100.034.02372730
ATCTCGT4050.033.8882337
AGACGAC4200.033.74934425
CGAGACG4350.033.62003723
ACGAGAC4350.033.62003722
GAGACGA4300.033.4877224
Produced by FastQC (version 0.11.2)