FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_64.35100000043f47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_64.35100000043f47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159261
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC2639316.572167699562353No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1956012.281726222992447No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG31001.9464903523147539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAA207.016904E-444.99748631
TGCCGTC27900.044.11044345
TCTGAAC250.002099010636.0092963
TGAGCGC250.0021022535.997998
GCCGTCT458.641273E-734.99804745
CATAATT300.005127625530.007754
ATCACAC300.00513550929.99832525
AGCGCAG300.00513550929.99832510
GTACTAA406.1108643E-428.1499461
ATGCCGT47100.026.08134744
GAAATCC450.001222316624.99860433
AACGGAA450.001222316624.99860412
GTATGCC49950.024.5481842
TATGCCG50100.024.51958843
GCCCACG50300.024.51155718
CCACGAG50300.024.51155720
CACGAGA50400.024.5075621
ACGAGAC50500.024.50358222
GCGAATC49900.024.48260133
TCTCGTA49750.024.46597138
Produced by FastQC (version 0.11.2)