FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_63.35100000043f2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_63.35100000043f2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112847
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC1189810.543479224082164No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT16991.505578349446596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG4510.3996561716306149No Hit
ATCGAAAAGAATCGAATCGAAAAGAATTGAATCGAATCGAAAAGAAACGAA2090.18520651856052886No Hit
GAGTATTAAGTGCTATCAATACCGCTTTATCAAAGAACAACATCAATATAG1760.15596338405097165No Hit
AATTAAGTGTCCTCCATGATCTACAGGATTAGTAACTATTCCGTCTTTTAT1700.15064645050377945No Hit
ATCTTTGAAGTATGACCATAAACAGTTCATTTCATTGTTCCCTGCCTCACT1520.1346956498622028No Hit
GTGTATTTCATCTGGTGTAAAATCTCAGCTGGTGTAAAACGCAGTGTATTT1500.1329233386798054No Hit
ATGATATTCGTATCTTGCGTGATATTGAGCAATATTACTCCACACAAATTG1390.12317562717661966No Hit
ATAACAAATGTGCATAAGGCATAGATACTGTAAACGGCTTGTATTCCTTTA1350.11963100481182487No Hit
AACAAGAGTGGAATGGAATGGAATGGAATGAAATGGAATGGAATGGAATGG1260.11165560449103655No Hit
TCTTGTATGAGTTCAGAATTGTTTTTGGGTGTAGGAAACAGAGATCTGAAG1210.10722482653504302No Hit
GAAGAAATTCATATGAAGAGACAGATTTTTTAGATCCATTTTGGCAAAAGA1180.1045663597614469No Hit
GAACAAAGTTGCACATGGTTCAGTGTAGCTTAACTAACAAAGCATTGGAAA1140.1010217373966521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAC206.991272E-445.0195161
TAGAGCA207.006572E-444.9995575
CGGAACA253.868623E-544.99955731
GAATGTC253.868623E-544.99955735
GGGTCAC207.006572E-444.99955714
CAGTTAG207.006572E-444.99955724
CACGACT207.006572E-444.99955714
CCGCTAT253.868623E-544.99955714
GCGCAAA207.006572E-444.99955731
TTGACGA207.006572E-444.99955729
ACTACCG253.868623E-544.99955710
ATCCACG207.006572E-444.99955711
CGCAGTG207.006572E-444.99955740
CATCGGA253.868623E-544.99955728
ACCGTTA207.006572E-444.99955742
TTTGACG207.006572E-444.99955728
ACGACTA207.006572E-444.99955715
CGCTTTA253.868623E-544.99955723
AGTGTAG253.868623E-544.99955722
ACGAAAC207.006572E-444.9995576
Produced by FastQC (version 0.11.2)