FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_62.35100000043f03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_62.35100000043f03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374551
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC19387751.762510312347324No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT5966115.92867192985735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG198285.293805115992215No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA5250.1401678276122611No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA4790.12788645605004392No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4580.12227974294555348No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4450.11880892054753557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGC302.1600226E-645.00467728
ACCAACG302.1600226E-645.00467727
CAACGCG302.1600226E-645.00467729
GGCGCAT253.88337E-545.00467332
AGGCGCA253.88337E-545.00467331
AAGGCGC253.88337E-545.00467330
GCGCATC253.88337E-545.00467333
CAAGGCC207.023595E-445.00467329
ACGCGAA351.208009E-745.00467331
AATGATC253.88337E-545.00467325
CAATGAG207.0282165E-444.998675
ATCTCAG207.0282165E-444.9986710
TATGCCC302.1620108E-644.99866545
TAGCTTG253.886434E-544.99866514
GCTTGCG253.886434E-544.99866516
TTGCGTA253.886434E-544.99866518
TGCCGTC200750.044.66243745
GCCGTCT1450.041.89530645
CGCGAAT403.447094E-739.3790932
AACGCGA403.447094E-739.3790930