FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_61.35100000043ee8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_61.35100000043ee8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137041
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC7293853.22348786129698No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2173515.860217015345773No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG55944.081990061368495No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG1470.10726716821972986No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATGC207.0115254E-445.00000428
AATCACG207.0115254E-445.00000436
ATCTAGT207.0115254E-445.00000437
TGCGAAT207.0115254E-445.00000432
ATGCGAA207.0115254E-445.00000431
CGAATCA207.0115254E-445.00000434
TGCCGTC78450.044.42638845
GCCGTCT1000.040.545
AAGCGAA301.1346816E-437.49999631
CCAATCT250.00210065136.034
AATCTAG250.00210065136.036
TCTAGTA250.00210065136.038
CACGTAT250.00210065136.039
ACGTATG250.00210065136.040
AGTATGC250.00210065136.041
TCACGTA250.00210065136.038
GAATCTA250.00210065136.035
GAATCAC250.00210065136.035
CGAGCGC250.00210065136.015
CCTTATA250.00210065136.01
Produced by FastQC (version 0.11.2)