FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_60.35100000043ece.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_60.35100000043ece.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217784
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC32081.4730191382287037No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT6510.29892003085626123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.042.8260845
ATGCCGT4750.037.4164744
AGGCGAA4750.036.47759231
TATGCCG4900.036.2710743
CAAGGCG4800.036.0976229
AAGGCGA4800.036.0976230
GTATGCC4950.035.90469442
GCGAATC4850.035.7254833
CGTATGC5000.035.54564741
CGAGACC4950.035.45834423
GGCGAAT4900.035.36092832
AATCTCG5050.035.19370736
ATCTCGT5100.034.8486737
ACGAGAC5100.034.41545522
CTCGTAT5200.034.1785139
CCAAGGC5100.033.9742328
TCTCGTA5250.033.85299738
TCGTATG5300.033.5336340
GAGACCA5250.033.4321524
CGAATCT5350.032.7997234