FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_6.3510000004380a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_6.3510000004380a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175605
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC27761.5808205916688023RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT4400.2505623416189744No Hit
ATATAATTCTCTTCTTCACATACAGCAGTTTAGATTCACAATACAACAAGC2840.16172660231770164No Hit
ATATATGATTGACCATAACACGATGTGATTACAATCAATTACAAAGCCAAA2580.14692064576748953No Hit
ATATAATTCTCTTCTTCACATACAGCGGTTTAGATTCACAATACAACAAGC2370.1349619885538567No Hit
GTATAGGTAAGCATTGCATGCTTATAAACCGATGTATGCAAGTAATAACTC2250.12812847014606646No Hit
CTTTACATCCTCATATTCATCTGTTAATTTGGCTTTGTAATTGATTGTAAT2190.12471171094217134No Hit
CTTATAAACCGATGTATGCAAGTAATAACTCTGTAAAAAAACGTTACAGAT1840.10478061558611657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3200.040.0763545
TACCCCC250.002102718735.99840540
AGTATTA250.002102718735.9984058
ATGCCGT3700.034.66062544
TATCTCG3800.034.34058436
GAATTGC3800.034.34058429
CGTATGC3750.034.19848341
TATGCCG3750.034.19848343
CCCACGA3750.034.19848319
GTATGCC3750.034.19848342
CGAGACG3800.033.74850523
CGAATTG3950.033.60610628
GACGAAT3900.033.46005626
GCCCACG3850.033.31021518
CTCGTAT3850.033.31021539
CACGAGA3850.033.31021521
TCGTATG3900.032.8831640
ACGAATT4100.032.37661427
GTATTAT352.802062E-432.1597481
GCAGTTT352.8114492E-432.14143425
Produced by FastQC (version 0.11.2)