FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_59.35100000043ea5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_59.35100000043ea5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208563
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC26421.266763519895667No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT10760.515911259427607No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2150.10308635759938244No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCA253.8804035E-544.9988025
TGCCGTC2900.044.22295845
ATGCCGT3600.034.9990744
TATGCCG3650.034.5196343
GTATGCC3650.034.5196342
CGTATGC3700.034.05314641
GGCGAAT3650.033.9032132
CAAGGCG3700.033.44505729
GCGAATC3750.032.99912333
AGGCGAA3750.032.99912331
TCGTATG3800.032.5649240
CGAGACC3850.032.14200223
AAGGCGA3850.032.14200230
CCAAGGC3950.031.3282828
ACGAGAC4050.031.11028122
CTCGTAT4000.030.93667639
AATCTCG4000.030.37419136
CGAATCT4000.030.37419134
CACGAGA4150.030.36063621
CCCACGA4250.030.17566719
Produced by FastQC (version 0.11.2)