FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_58.35100000043e8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_58.35100000043e8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26420
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC3801.4383043149129449No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC850.32172596517789553No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT410.15518546555639667No Hit
GGAGGAGGAGAAATTGAAGTGCTTTTGATAAGTGCACAGAAGAAAGGGAAA400.1514004542013626No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA330.12490537471612415No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA300.11355034065102196No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC290.10976532929598788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC650.041.53846445
GTATGCC800.036.56250442
TATGCCG800.036.56250443
CGTATGC800.036.56250441
ATCTCGT751.8189894E-1236.037
GCCCACG751.8189894E-1236.018
CCCACGA751.8189894E-1236.019
TCTCGTA751.8189894E-1236.038
ACGAGAC751.8189894E-1236.022
ATGCCGT751.8189894E-1236.044
GAATCTC751.8189894E-1236.035
AAGGCGA751.8189894E-1236.030
TCGTATG751.8189894E-1236.040
CAAGGCG751.8189894E-1236.029
CTCGTAT751.8189894E-1236.039
CGAGACC701.8189894E-1135.35714323
CCGAGCC850.034.41176214
TCCGAGC850.034.41176213
AATCTCG801.8189894E-1233.75000436
CCACGAG801.8189894E-1233.75000420
Produced by FastQC (version 0.11.2)