FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_57.35100000043e61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_57.35100000043e61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34974
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC754321.567450105792872No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT9802.802081546291531No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG2650.7577057242523018No Hit
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC1260.3602676273803397RNA PCR Primer, Index 46 (95% over 22bp)
GAAGAGAATCGATTAGAATCGAAATGAAAAGAATCGTATTGAATCGAATCG560.16011894550237318No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT470.13438554354663465No Hit
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT460.1315262766626637RNA PCR Primer, Index 46 (95% over 21bp)
GATATACATCGTGCAATGAACCAAGAAGAAAGACTCAGTAGATACCAACTG450.12866700977869275No Hit
TTGTGTATCCTTTTGCTTGTTTTTTGTTGTGATAGTCTTTGAGTTACTCAA430.12294847601075085No Hit
GTTTTGATTAGTAAGGACTGAAACTATGGAGTCATCTTTTTTCACATCATT410.11722994224280894No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG410.11722994224280894No Hit
TATCAAGCTCTTTGTAGATTTCACACTAACCGTCTTGGATACTTTTGTTGT370.10579287470692514No Hit
GTGTTTATTTCCGATATTCGTAACTGTAAAAGTAAAGAAGCCGAAAGCAAA350.10007434093898325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8250.042.045
CGTCTTC356.0762686E-638.57142645
ATGCCGT9500.036.47368244
CCCACGA9750.035.5384619
CGAGACC9750.035.5384623
AGACCAA9750.035.5384625
GACCAAG9750.035.5384626
GAGACCA9750.035.5384624
CGTATGC9700.035.48969341
TCGTATG9700.035.48969340
ATCTCGT9650.035.44041437
AGCCCAC9800.035.35714317
GCCCACG9800.035.35714318
TATGCCG9800.035.35714343
ACCAAGG9800.035.35714327
CACGAGA9800.035.35714321
CCAAGGC9800.035.35714328
GTATGCC9750.035.3076942
CTCGTAT9750.035.3076939
TCTCGTA9700.035.25773238