Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pa_57.35100000043e61.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 34974 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC | 7543 | 21.567450105792872 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT | 980 | 2.802081546291531 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG | 265 | 0.7577057242523018 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTTC | 126 | 0.3602676273803397 | RNA PCR Primer, Index 46 (95% over 22bp) |
GAAGAGAATCGATTAGAATCGAAATGAAAAGAATCGTATTGAATCGAATCG | 56 | 0.16011894550237318 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT | 47 | 0.13438554354663465 | No Hit |
TATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCTT | 46 | 0.1315262766626637 | RNA PCR Primer, Index 46 (95% over 21bp) |
GATATACATCGTGCAATGAACCAAGAAGAAAGACTCAGTAGATACCAACTG | 45 | 0.12866700977869275 | No Hit |
TTGTGTATCCTTTTGCTTGTTTTTTGTTGTGATAGTCTTTGAGTTACTCAA | 43 | 0.12294847601075085 | No Hit |
GTTTTGATTAGTAAGGACTGAAACTATGGAGTCATCTTTTTTCACATCATT | 41 | 0.11722994224280894 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG | 41 | 0.11722994224280894 | No Hit |
TATCAAGCTCTTTGTAGATTTCACACTAACCGTCTTGGATACTTTTGTTGT | 37 | 0.10579287470692514 | No Hit |
GTGTTTATTTCCGATATTCGTAACTGTAAAAGTAAAGAAGCCGAAAGCAAA | 35 | 0.10007434093898325 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 825 | 0.0 | 42.0 | 45 |
CGTCTTC | 35 | 6.0762686E-6 | 38.571426 | 45 |
ATGCCGT | 950 | 0.0 | 36.473682 | 44 |
CCCACGA | 975 | 0.0 | 35.53846 | 19 |
CGAGACC | 975 | 0.0 | 35.53846 | 23 |
AGACCAA | 975 | 0.0 | 35.53846 | 25 |
GACCAAG | 975 | 0.0 | 35.53846 | 26 |
GAGACCA | 975 | 0.0 | 35.53846 | 24 |
CGTATGC | 970 | 0.0 | 35.489693 | 41 |
TCGTATG | 970 | 0.0 | 35.489693 | 40 |
ATCTCGT | 965 | 0.0 | 35.440414 | 37 |
AGCCCAC | 980 | 0.0 | 35.357143 | 17 |
GCCCACG | 980 | 0.0 | 35.357143 | 18 |
TATGCCG | 980 | 0.0 | 35.357143 | 43 |
ACCAAGG | 980 | 0.0 | 35.357143 | 27 |
CACGAGA | 980 | 0.0 | 35.357143 | 21 |
CCAAGGC | 980 | 0.0 | 35.357143 | 28 |
GTATGCC | 975 | 0.0 | 35.30769 | 42 |
CTCGTAT | 975 | 0.0 | 35.30769 | 39 |
TCTCGTA | 970 | 0.0 | 35.257732 | 38 |