FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_56.35100000043e48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_56.35100000043e48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272719
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC252029.241013644080537No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT54912.013427740641466No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG19420.7120882666774225No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC7670.2812418643365516No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7660.28087518654732524No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC6710.24604079657082933No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5950.21817328458963256No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4650.17050517199021703No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3480.12760387065074305No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3470.1272371928615168No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT3300.12100367044467016No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3250.1191702814985388No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3240.11880360370931252No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC3040.11147004792478706No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2870.1052365255079404No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2840.10413649214026158No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2830.10376981435103533No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2800.10266978098335651No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT2800.10266978098335651No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2750.10083639203722512No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA2730.10010303645877258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGA207.023955E-444.99981733
TACCGTG207.023955E-444.99981732
TGCCGTC29050.043.83802845
GCCGTCT556.002665E-1140.90892445
GGGGAGT250.002104339435.99985519
ATGCCGT35700.035.67212344
TATCTCG36750.034.16312436
CCTATCT36750.034.16312434
CGAGACG37700.034.07811423
CTATCTC36800.034.0555735
ACCTATC36950.034.03910433
CTCGTAT37300.034.02130539
TATGCCG37450.034.00520343
TCTCGTA37300.033.90066538
ACGAGAC38000.033.86828222
ATCTCGT37350.033.8552837
CGTATGC37550.033.8547241
TCGTATG37500.033.83986340
CGATAAC37700.033.83938628
GTATGCC37650.033.82456242
Produced by FastQC (version 0.11.2)