FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_55.35100000043e2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_55.35100000043e2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147978
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC2313115.631377637216342No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT118868.032275067915501No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG18621.2582951519820513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23950.044.7174945
GTGTGAT301.1352214E-437.49943511
TCCCACT250.002094381736.0238081
TCACCCA250.002101347835.9994623
TTCGTAT250.002101347835.9994644
TCAGGCA250.002101347835.9994611
GACCATC250.002101347835.9994633
AGTGTGA352.8088395E-432.14237210
GTCTAGG300.00511636730.019841
GTATAGA300.00511636730.019841
ACGTTGA300.0051333229.9995522
GTTCGAT300.0051333229.9995528
CGATATC300.0051333229.9995531
TGTCCTA300.0051333229.9995511
TCAAACG300.0051333229.999558
ATGCCGT37200.028.72940644
GTCTAAA406.1168615E-428.14361
ATCATAC406.141117E-428.1245773
CTTACAA406.141117E-428.12457715
TATGCCG40200.026.5854243
Produced by FastQC (version 0.11.2)