FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_54.35100000043e04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_54.35100000043e04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214777
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC12330657.41117531206786No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT4132519.240887059601352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG114895.349269242051058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT207.0222554E-444.99767339
AGCTCCG207.0222554E-444.99767310
GATGACC207.0222554E-444.99767329
GCTCGTA207.0222554E-444.99767338
AGACGAC207.0222554E-444.99767325
AGACGAA207.0222554E-444.99767325
CGAATGC207.0222554E-444.99767341
GACGATG207.0222554E-444.99767326
GCTCCGA207.0222554E-444.99767311
ACCTAGC207.0222554E-444.99767333
CGATGAC207.0222554E-444.99767328
AATGCCG207.0222554E-444.99767343
ACAGCTC207.0222554E-444.9976738
GACCTAT253.881368E-544.9976732
TGCCGTC129450.044.66744645
TCTTTAT250.00209903236.0149081
ACACAGC250.002101433736.006526
CAGCTCC250.00210383835.9981359
ATCTCGA250.00210383835.99813537
CACGTAT250.00210383835.99813539
Produced by FastQC (version 0.11.2)