FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_53.35100000043de9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_53.35100000043de9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143369
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC6581045.90253123060076No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT100547.012673590525148No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG42252.94694111000286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCGA207.014493E-444.99756211
TGCCGTC69000.044.3454245
ATGCCGT81350.037.613244
TACACAG301.1329762E-437.5110475
AGCTCCG250.002101534735.99804710
TATCTCG84900.035.8019536
ATCTCGT85000.035.75982737
CCTATCT85000.035.70688634
AACCTAT85400.035.69771232
ACGAGAC86100.035.69492722
CTATCTC85050.035.68589435
TCTCGTA85250.035.68134738
CCCACGA86200.035.6796219
TAACCTA85450.035.67682331
CTCGTAT85400.035.67136839
CACGAGA86350.035.6697521
GCCCACG86300.035.66434518
CCACGAG86300.035.66434520
CGAGACG86000.035.65794823
ATAACCT85500.035.6559630
Produced by FastQC (version 0.11.2)