FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_52.35100000043dcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_52.35100000043dcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268156
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC76772.8628857829024894No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT30791.1482122346693717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG4960.18496695953101927No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4300.1603544205611659No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2880.10740017005026925No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2820.10516266650755529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7850.044.42233345
CCCCCCG250.002105262635.99641842
CGGAGGT352.8162939E-432.1396619
ATGCCGT10950.031.84614844
TGTACAG300.005133431430.0082075
ACCTATC11950.029.3749433
GTATGCC11950.029.36946542
TATGCCG11900.029.30380843
TATCTCG11950.029.18119836
TCGTATG11950.029.18119840
CTCGTAT12000.029.0596139
CCTATCT12100.028.82482134
AACCTAT12200.028.77299532
CGTATGC12200.028.76763241
TCTCGTA12150.028.7008538
CGAGACG12300.028.53374523
TAACCTA12350.028.42352531
ACGAGAC12350.028.41822422
ATCTCGT12300.028.35083637
GACGATA12500.028.2624626