FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_51.35100000043da6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_51.35100000043da6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180261
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC3038416.85555943881372No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT130247.225079190728999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG23951.3286290434425638No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10330.5730579548543501No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA8830.489845279899701No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC7970.44213667959236885No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT7450.4132896189414238No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA7120.39498283045140103No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC6730.3733475349631923No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC6390.35448599530680514No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA5330.2956823716721865No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5230.29013486000854316No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC5140.28514209951126424No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC4890.27127332035215607No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC4790.26572580868851275No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4480.24852852253121863No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4260.23632399687120342No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4200.23299548987301744No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3760.20858643855298703No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3690.2047031803884368No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA3690.2047031803884368No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3600.19971041989115781No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3350.18584164073204965No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC3300.183067884900228No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA3200.17752037323658473No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2990.16587059874293386No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG2950.16365159407747654No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC2880.15976833591292625No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT2790.15477557541564732No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA2720.15089231725109703No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC2660.14756381025291107No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2570.14257104975563212No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT2530.14035204509017482No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2390.1325855287610742No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG2390.1325855287610742No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC2380.13203077759470988No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC2370.13147602642834558No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC2370.13147602642834558No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT2340.12981177292925258No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT2320.12870227059652392No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG2310.12814751943015962No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCCTGTCTCTTATACACATCTCC2300.1275927682637953No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA2300.1275927682637953No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA2290.12703801709743096No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC2270.1259285147647023No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2170.12038100310105902No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACT2130.11816199843560171No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT2110.11705249610287306No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC2000.11095023327286546No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1960.10873122860740815No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC1930.10706697510831516No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA1920.10651222394195084No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT1890.10484797044285786No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC1870.1037384681101292No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG1860.10318371694376487No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC1850.10262896577740055No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA1830.1015194634446719No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA1810.10040996111194325No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAA207.0174504E-445.020
GCAAGAA207.0174504E-445.019
ACGCAAG207.0174504E-445.017
CATCTCG207.0174504E-445.036
CAATAAC207.0174504E-445.012
CGCAAGA207.0174504E-445.018
TGCCGTC32200.044.37111745
TTTTGTA800.036.56252
TGTGACA250.002102408336.07
AACTCTG250.002102408336.05
AATAACG250.002102408336.013
GCCGTCT458.6459295E-735.045
TTTGTAC900.032.53
TAAAAGT352.811013E-432.1428571
ATGCCGT45100.031.62971144
TTGTACT950.030.7894764
TGTACTC901.2732926E-1130.05
GAATGTG300.005135885429.9999984
AAGAAAC300.005135885429.99999821
AAGGAAT300.005135885429.9999981
Produced by FastQC (version 0.11.2)