FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_48.35100000043d3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_48.35100000043d3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282891
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC169615.995595476703041RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT76462.702807795228551No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC13600.48075053642569043No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA11360.40156809513204733No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC11360.40156809513204733No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA11180.39520522038523676No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG9380.33157647291713066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6430.22729602567773452No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA6020.21280281097666595No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5950.21032835968623959No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC5340.18876528415538138No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC5330.18841179111389192No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5180.18310939549154975No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA4510.1594253617117547No Hit
AAGGAATGTGACAATAACGCAAGAAACCTGTCTCTTATACACATCTCCGAG3820.1350343418489807No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA3190.11276428023514357No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3160.11170380111067514No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3070.10852236373726984No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC3060.10816887069578036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG2840.10039202378301183No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATC207.024802E-444.99929415
TGCCGTC18700.044.397745
ACACACG403.44633E-739.37438212
GCAATTC301.1378527E-437.4994138
GGCTCGT301.1378527E-437.49941321
GGAATGT751.8189894E-1236.00583
GAGGATC250.002104591635.99943533
CGTCTTG250.002104591635.99943534
CGGATCA250.002104591635.99943516
GCCGTCT250.002104591635.99943545
TCTAACG458.670195E-734.9994528
ACTGCGT401.5565933E-533.7494710
ACGCAAG606.3973857E-933.7494717
CTGCGTA401.5565933E-533.7494711
CGCAAGA606.3973857E-933.7494718
ATAACGC855.456968E-1231.76420614
ATGCCGT26750.031.03689444
TATGCCG27650.030.02665143
CACTGCG453.4820165E-529.9995299
TAGCACC300.00514117229.9995294
Produced by FastQC (version 0.11.2)