FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_46.35100000043cf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_46.35100000043cf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231339
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC4720620.405552025382665RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT2842612.287595260634825No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG46782.022140668023982No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGC3110.1344347472756431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGA207.0219254E-444.9995718
CACGGAT207.0219254E-444.9995719
CTCCCGC207.0219254E-444.999579
GTCTCCG207.0219254E-444.9995711
CGTCTCC207.0219254E-444.9995710
GTAGGTC207.0219254E-444.9995720
CGCGAGT207.0219254E-444.9995720
CGTTATC207.0219254E-444.999572
CGCCTTC207.0219254E-444.9995711
AATGCGG207.0219254E-444.9995724
CCCCGGT302.1581036E-644.9995711
ACGTCTC207.0219254E-444.999579
CCCGGTT302.1581036E-644.9995712
GCCACGT207.0219254E-444.9995739
ACCCCGG302.1581036E-644.9995710
GCACTCT207.0219254E-444.9995742
TGCACTC207.0219254E-444.9995741
GGCTCAA207.0219254E-444.9995723
GCTCCCG207.0219254E-444.999578
TGCCGTC50800.044.55665645
Produced by FastQC (version 0.11.2)