FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_45.35100000043cdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_45.35100000043cdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163765
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC9493857.97209415931365RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT2782816.992641895398894No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG68894.206637559918176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG207.018843E-444.99572836
ACCGAAT207.018843E-444.99572832
TACCGAA207.018843E-444.99572831
TGCCGTC98500.044.49323745
GCCGTCT1050.038.56776445
GACCATA356.219587E-638.56776426
AGCCCAA301.1362872E-437.4964417
TCAGCAT250.002096528436.0185783
CATACCG250.002102827235.99658229
ATGCCGT127600.034.32863644
TATGCCG135350.032.3297743
GAGCCCA136200.032.29318616
CGAGCCC136250.032.28133815
AGCCCAC135900.032.2651517
CCGAGCC136350.032.2576614
TCCGAGC136300.032.25298713
CTCCGAG136350.032.2411612
GCCCACG135800.032.23920418
CCCACGA135750.032.23450519
CACGAGA135900.032.23203721
Produced by FastQC (version 0.11.2)