FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_40.35100000043c4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_40.35100000043c4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179912
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2721315.12572813375428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT2232312.407732669304995No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG36592.0337720663435457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG207.010579E-445.0088961
CTACGGT253.8731276E-545.0088923
GGTACTC207.02019E-444.9963877
GTAGGTT207.02019E-444.99638736
TGCCGTC28850.044.29453745
CAAGGGT356.2105137E-638.5790564
TAGGTTG250.00210035836.0071145
GCTACGG250.00210035836.0071142
TAGTATG250.00210035836.0071144
GTCAGGC250.00210322735.9971116
GTCATGA458.651241E-734.99719216
TCGTCAT458.651241E-734.99719214
GGTTACA401.5511865E-533.7566733
ATTCGTC401.5541433E-533.74728812
GCCTTAC352.812316E-432.14027438
GAATCCT352.812316E-432.14027420
TACGGTA300.005130893630.005934
AGTATGG300.005130893630.005935
CCCACAC300.005130893630.005933
CGGTACT300.005130893630.005936
Produced by FastQC (version 0.11.2)