FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_39.35100000043c20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_39.35100000043c20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190575
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC67333.5329922602649875No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT11760.6170798898071626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.18942673488128034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG2560.13433031614849797No Hit
GAAGGAGAAGGAGAAAAAGGATAAGGAGGAAAAGGAGAAGAAGGATAAGGA2190.11491538764266038No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2180.1143906598452053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.11124229306047487No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2050.10756919847828939No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.10494555949101404No Hit
GAGGAAAAGGAGAAGAAGGATAAGGAAAGGAAGGAAAAGGAGAAGAAGGAT1940.10179719270628361No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1940.10179719270628361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.10022300931391841No Hit
GGATAAGGAAAGGAAGGAAAAGGAGAAGAAGGATAAACTGGAAAAGGAAAA1910.10022300931391841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7450.043.1879245
ATGCCGT8650.037.45664644
TGCGACC8750.036.7714329
TATGCCG8850.036.6101743
CGTATGC8800.036.56250441
CTGCGAC8800.036.56250428
CTCGTAT8850.036.3559339
TCTCGTA8850.036.3559338
TCGTATG8850.036.3559340
GACTGCG8850.036.3559326
ACTGCGA8900.036.15168427
GTATGCC8900.036.15168442
CCTATCT8900.036.15168434
CCAGGTT250.002102709836.038
GATATAA250.002102709836.02
CCAGTCC250.002102709836.020
CTGTGCT250.002102709836.09
AGACTGC8950.035.94972225
GAGACTG8900.035.89887624
CGAGACT8850.035.84745823
Produced by FastQC (version 0.11.2)