FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_35.35100000043b9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_35.35100000043b9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126120
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2073916.443862987630826No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT1278010.133206470028544No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG14441.144941325721535No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG1690.13399936568347603No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAGG206.985669E-445.0309523
TGCCGTC21900.043.86522345
CTCTACA250.002092936836.024761
GAAATCC250.00210110835.9961925
GTGCTTC300.00511286730.0206366
GTGCTAC300.00511286730.0206361
TCTGAGT300.005132753429.99682625
GAGTAAA300.005132753429.99682611
GTGAGTA300.005132753429.9968269
GAGTTGT300.005132753429.9968267
GCCGTCT554.1298154E-628.63333545
CAATTTG406.139297E-428.12202614
GAGTACG654.82014E-727.6893812
ATGCCGT34900.027.52574244
TATCTCG36450.026.04662736
TCTCGTA36550.026.03691938
TATGCCG36900.026.03383443
CCTATCT36650.026.02726234
GACCTAT36850.026.00810232
TCGTATG36900.025.97286440
Produced by FastQC (version 0.11.2)