FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_32.35100000043b4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_32.35100000043b4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218818
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC142696.520944346443162No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT30141.3774003966766903No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG5000.2285003975906918No Hit
TCTAAGGAGAGAGTTATAAAGATGGGAAAGAAGAAAACCTGTACTGTTAGA2970.13572923616887092No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA207.011621E-445.0118875
TGACTCG207.011621E-445.0118875
GCCTCTA302.1568922E-645.00160221
CGTATGG207.0195243E-445.001622
CCGTATG207.0195243E-445.001621
ACGGCAG207.0195243E-445.001625
TACGACT207.0195243E-445.001643
AGCGAGC207.0195243E-445.001640
ATTGGAC406.7702786E-945.001617
TGGTCAC207.0195243E-445.001625
GTCGGTT207.0195243E-445.001621
AGAGATC207.0195243E-445.001620
AACCGTG253.8848484E-544.9913187
TAACGTC207.0274353E-444.99131415
GCTTCGA207.0274353E-444.99131445
CGCTACA207.0274353E-444.9913149
CCCCCCA207.0274353E-444.99131414
ACGCTAC207.0274353E-444.9913148
ACCCCCC207.0274353E-444.99131413
TGCCGTC14450.043.43452545