FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_30.35100000043b07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_30.35100000043b07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353681
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC18934953.53666156791007No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT3930911.11425267401981No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG136043.8464039628931155No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA6440.18208498618811866No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA6290.17784387626137677No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC5940.16794795309897903No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC5740.16229313986332317No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5370.15183173537735983No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4330.12242670655194934No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT3970.11224804272776882No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT3680.10404856353606783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCT207.0181745E-445.0110324
ATCTAGT207.0279615E-444.99830237
GAATCTA207.0279615E-444.99830235
TCTAGTA207.0279615E-444.99830238
AGTATGC207.0279615E-444.99830241
TGCATAA253.886176E-544.99830231
CCGTATG302.1617852E-644.99830240
CCCGTAT302.1617852E-644.99830239
CGAATCT253.886176E-544.99830234
TAATCTC253.886176E-544.99830235
TGCCGTC195750.044.67647645
CTTTCAG451.9224899E-839.99849329
GCCGTCT1350.039.99849345
ACACATT356.2255403E-638.5808836
TTGCATA301.13857706E-437.49858530
GACCTTT601.546141E-1037.49858526
ACCTTTC601.546141E-1037.49858527
ATGCCGT237350.036.8555744
AAGAGTA250.002102609336.0088232
TTTCAGA504.8641596E-835.99864230
Produced by FastQC (version 0.11.2)