FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_3.3510000004379f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_3.3510000004379f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312050
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC26740.8569139560967793RNA PCR Primer, Index 27 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC6650.2131068738984137No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT4720.15125781124819743No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA3660.11728889601025476No Hit
TATTAGCTCTAGAATTACTACGGTTATCCGAGTAGTAGTTACCATCAAACA3370.10799551353949688No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC3320.10639320621695242No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG3270.10479089889440796No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG207.0161396E-445.012024
TGCCGTC3650.035.75151445
ATGCCGT3850.033.8942944
CGTATGC3950.033.605841
TCGTATG3950.033.0362140
TATGCCG4000.032.62325743
CTCGTAT4100.032.3763239
TAACCGG352.8165834E-432.1411444
GTATGCC4100.031.82756642
GCTATCT4200.031.60545534
TGCTATC4200.031.60545533
ATTGCTA4300.031.39367131
CCCACGA4300.030.87044319
TCTCGTA4400.030.6801838
GAATTGC4400.030.6801829
CACGAGA4350.030.51561421
GCCCACG4400.030.16884418
ACGGACG300.00514292329.998417
CGGACGA300.00514292329.998418
AATTGCT4450.029.82986630
Produced by FastQC (version 0.11.2)